The Proteome of Filter-Grown Caco-2 Cells With a Focus on Proteins Involved in Drug Disposition

Abstract

Green tea leaf aqueous extracts of varying volumes (5ml, 10ml, 15ml, 20ml, 25ml) are
outlined for the green synthesis of silver nanoparticles using a 1Mm silver nitrate solution.
UV-visible absorption spectroscopy, FTIR, SEM, and HPLC are utilized to evaluate
synthesized nanoparticles. The synthesis was predicated on the phenomenon of surface
plasmon resonance, as determined by UV- spectra at 200-400 nm, which confirmed the
presence of silver nanoparticles. The Fourier-transform infrared (FTIR) spectrum confirms
the presence of Amine, Alcohol, Aldehyde, Aromatic, C-H bending, and C-O bending
functional groups as concentrations of green tea leaf extracts increase. The Scanning Electron
Microscopy (SEM) analysis reveals a helical structure of silver nanoparticles ranging in size
from 5 to 100 nm, which exhibits good antioxidant and antimicrobial properties against
pathogens such as E. coli and Staphylococcus aureus, as well as good antimicrobial activity